STEMCELL Technologies STEMdiff Human iPSC-Derived Neural Progenitor Cells
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- 新製品
Human iPSC-Derived Neural Progenitor Cells (ST-200-0620) は、ただちに使用可能な高品質のヒト神経前駆細胞（NPC）です。本品の使用により、神経ワークフローを自信を持って開始できます。
健康な女性ドナー末梢血単核細胞（PBMC）由来のヒト人工多能性幹細胞（iPSC）コントロールラインであるSCTi003-Aから分化した、凍結保存された中枢神経系（CNS）タイプの前駆細胞です。これらのヒトNPCは多能性で、解凍直後から使用でき、STEMdiff™キットを用いて前脳ニューロン、中脳ニューロン、アストロサイトなど様々な中枢神経系細胞への下流分化を行うのに適しています。
本品のNPCは高い品質を確保するため、広範囲に試験された堅牢なSCTi003-A株から、無血清のSTEMdiff™ SMADi Neural Induction Kitを用いて分化しました。NPCはSTEMdiff™ Neural Progenitor Mediumによる拡大培養でスケールアップでき、大量に細胞を必要とするワークフローのコスト削減を可能にします。増殖したNPCは、STEMdiff™ Neural Progenitor Freezing Mediumを用いて凍結保存することで、実験スケジュールを柔軟に変更することができます。
本品は研究用製品（RUO）で、学術・商業研究用としての承認を受けています。血液サンプルは、治験審査委員会（IRB）またはその他の規制当局が承認した同意書およびプロトコルを用いて倫理的に提供されています。
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
ヒトiPS細胞株 SCTi003-Aから分化した神経前駆細胞（NPC）です
- 高度に特性化されたコントロールiPS細胞 SCTi003-A株由来の高品質なNPCです
- 解凍後すぐに、STEMdiff™ Neural Progenitor Mediumで増殖できます
- 高度に特性化されたNPCを中間体として分化ワークフローを開始することで、時間を節約できます
- STEMdiff™ 神経培養系を用いて、前脳ニューロンやアストロサイトに分化できます
- 同一の遺伝的背景で作製したニューロン-アストロサイト共培養によって関連性を確保できます
ご注意
ソースセルバンクのドナー詳細および細胞品質特性については、以下のSCTi003-Aの情報をご参照ください。
コントロールに最適な凍結ヒトiPS細胞株
SCTi003-Aは、αβT細胞に由来し、VDJ配列の再構成を受けています。核型的に安定であり、3胚葉分化能を示し、未分化細胞マーカーを発現し、非組み込み型技術によりリプログラミングされています。hPSCreg®に登録されており、コミュニティ基準に基づく倫理的・生物学的適合性が保証されます。詳細については、ロット別の検査証明書（CoA）およびiPSC株に関するよくある質問もご参照ください。
データ紹介
Figure 1. Human iPSC-Derived Neural Progenitor Cells Exhibit High-Quality Morphology Characteristic of Multipotent Central Nervous System Progenitor Cells
Cryopreserved Human iPSC-Derived Neural Progenitor Cells were thawed and plated onto Corning® Matrigel®-coated plates at 200,000 cells/cm². NPCs were incubated for 24 hours in STEMdiff™ Neural Progenitor Medium at 37℃ and subsequently analyzed by brightfield microscopy. NPCs display the small, teardrop-shaped morphology expected for NPCs. (A) 4X magnification, (B) 10X magnification. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.
Figure 2. Human iPSC-Derived Neural Progenitor Cells Express Characteristic Markers
Human iPSC-Derived Neural Progenitor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. The NPCs express neural progenitor markers (A) SOX1 and (B) PAX6 with low expression of (C) βIII-TUB. (D) In addition, they display the expected small, teardrop-shaped morphology. (E) The percentage expression of these markers were quantified. Neural progenitor markers PAX6 and SOX1 were found to be expressed in 90% of NPCs, while mature neuronal marker βIII-TUB was expressed in less than 10% of NPCs. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.
Figure 3. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Forebrain Neurons
Forebrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Forebrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Forebrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Forebrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting forebrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (magenta), (C) but not astrocyte marker GFAP (green). (D) Nuclei are labeled with DAPI (gray). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.
Figure 4. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Midbrain Neurons
Midbrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Midbrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Midbrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Midbrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting midbrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (red) and (C) dopaminergic neuron marker TH (green), (D) but not astrocyte marker GFAP (magenta). (D) Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.
Figure 5. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Astrocytes
Astrocytes were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Astrocyte Differentiation Medium. NPCs were cultured for 21 days at 37℃ in STEMdiff™ Astrocyte Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Astrocyte Maturation medium for seven days. Resulting cells were processed for immunocytochemistry. (A) The resulting astrocyte cultures contain a population of cells expressing (B) astrocyte marker S100β (green) and (C) GFAP (red), but not neuronal marker DCX (magenta). Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.
Figure 6. Human iPSC-Derived Neural Progenitor Cells Can Be Expanded for Multiple Passages
Human iPSC-Derived Neural Progenitor Cells were thawed and maintained in STEMdiff™ Neural Progenitor Medium at 37℃ and passaged roughly once every seven days for a total of five weeks. The average fold increase of NPCs was 2.8 ± 0.5 (mean ± SEM) per passage, demonstrating cells can be effectively expanded.
