STEMCELL Technologies STEMdiff STEMdiff Neural Progenitor Medium
- 研究用
STEMdiff™ Neural Progenitor Mediumは、STEMdiff™ Neural Induction Medium(ST-05835)を使用してヒトES/iPS細胞から誘導された神経前駆細胞(NPC)を増殖するためのdefinedな組成の無血清培地です。この培地中で培養されたNPCは、継代当たり3〜5倍に増殖させることができ、自発的神経分化を最小に抑えながら、少なくとも10継代は培養できます。
製品の特長

Figure 1. Morphology and Marker Expression of Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium
(A) Typical NPC morphology is observed in cultures (shown at day 6 of passage 1). (B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). B-D were taken at the same magnification.

Figure 2. Expansion of Neural Progenitor Cells in STEMdiff™ Neural Progenitor Medium
NPCs cultured in STEMdiff™ Neural Progenitor Medium can be expanded to generate a large number of cells. Three- to five-fold expansion can be achieved upon each passage. NPCs were derived using STEMdiff™ Neural Induction Medium and passaged once a week on average. n = 6.

Figure 3. Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium Show Minimal Spontaneous Neuronal Differentiation
Passages 1 (A) and 3 (B) of a representative NPC culture maintained in STEMdiff™ Neural Progenitor Medium. Cells were immunolabeled with SOX1 (red) to identify NPCs, and class III β-tubulin (green) to identify neurons. Spontaneous neuronal differentiation is low in NPC cultures maintained in STEMdiff™ Neural Progenitor Medium. A and B were taken at the same magnification.

Figure 4. Neural Progenitor Cells Maintained in STEMdiff™ Neural Progenitor Medium can Differentiate into Neurons and Astrocytes
When directed according to published protocols, NPCs can differentiate into neurons (A, class III β-tubulin shown in red) and astrocytes (B, GFAP shown in red). Nuclei are counterstained with DAPI (blue).
関連リンク
ヒトES/iPS細胞における品質管理の考え方:細胞品質管理のタイミング
「mTeSR Plus」新発売:培地交換週2回で高品質なES/iPS細胞を!
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神経細胞を生きたまま標識できる蛍光プローブ NeuroFluor NeuO / NeuroFluor CDr3
ヒトES/iPS細胞からの分化 研究用培地(STEMCELL Technologies社)
技術資料
アプリケーションノート
資料動画
- 製品紹介:TeSRTM - 無限の可能性
- 製品紹介:STEMdiffTM Neural System - ヒトES/iPS細胞由来神経モデル
- 動画マニュアル: STEMdiffTM Neural Induction MediumによるヒトES/iPS細胞からの神経前駆細胞分化(EB法)
- 製品紹介:STEMdiffTM - ヒトES/iPS細胞から様々細胞へ分化
- Maintaining and Assessing High-Quality hPSC Cultures
- Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
- Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability
- Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease
- Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
- STEMdiffTM Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
製品関連文献
- Bell S et al. 2018. Disruption of GRIN2B Impairs Differentiation in Human Neurons. Stem Cell Reports.11(1):183-196.
- Belle K et al. 2017. Generation of disease-specific autopsy-confirmed iPSCs lines from postmortem isolated Peripheral Blood Mononuclear Cells. Neurosci Lett.637:201-206.
- Gabriel E et al. 2017. Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids. Cell Stem Cell. 20(3):397-406.e5.
- Maricque BB et al. 2017. A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells. Nucleic Acids Res. 45(4):e16.
- Zhou P et al. 2016. Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions. Biomaterials. 87:1-17.
よくある質問
- STEMdiff Neuralはガラス底で使用できますか?
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本製品のガラス底96ウェルプレートでの使用につきまして、STEMCELL Technologies社では評価しておりませんが、poly-L-ornithine (for neurons) およびMatrigel hESC-qualified Matrix (for astrocytes)でプレートをコーティングできれば、お試しいただけると考えております。
ガラス面への細胞接着が上手くいくかどうかは、ガラスの種類、ラミニンの濃度、poly-L-ornithineを希釈するバッファーなど、いくつかの因子に依存します。
STEMCELL Technologies社のプロトコールを元に、ガラスに最適な条件をまずはご検討ください。開く閉じる - STEMdiff APEL2 MediumでヒトiPS細胞から血球分化する際に、PFHMの添加が必要ですか?
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ヒトiPS細胞からの血球分化の際には5% PFHM (Gibco® PFHM-II, Thermo Fisher Catalog #12040077)の添加が必要な可能性が高いです。
ただし、予め条件検討をお願いいたします。
添加する場合は5 mLのPFHMを100 mLのAPEL2に加えてください。
※ ヒトiPS細胞から造血前駆細胞へ分化させる培地「STEMdiff Hematopoietic Progenitor」(製品コード:ST-05310)もございますのであわせてご検討ください。開く閉じる