STEMCELL Technologies PancreaCult PancreaCult Mouse Organoid Medium

  • 研究用

PancreaCult™ Organoid Growth Medium (Mouse)は、マウス膵外分泌オルガノイドを樹立し維持するための、definedな組成の無血清培地です。これらのオルガノイドあるいは「ミニ膵臓」は、膵臓細胞の生物学、疾患、がんを研究するためのin vitro器官培養系を提供します。PancreaCult™で培養したオルガノイドは、膵臓幹細胞(LGR5)、前駆細胞(PDX1、SOX9)、および膵管細胞(CAR2、MUC1、KRT19)のマーカー遺伝子を発現する上皮を特徴とします。膵オルガノイドは、3〜6日ごとに継代し長期に維持することができ、凍結保存も可能です。


2018/05/14 12:00の製品情報






  • 簡易な in vitro 培養系:7日以内で膵臓オルガノイドの形成が可能
  • STEP-BY-STEPの詳細なプロトコール:障害モデルや膵管のハンドピッキングおよび細胞ソーティングなど不要 
  • シンプルな2-コンポーネント組成:成分が明確な無血清培地
  • フレキシブルなプロトコール:マトリックスドームまたは懸濁液でオルガノイドの形成・維持が可能 


  • 膵幹細胞・膵管上皮細胞研究
  • 膵臓がん・疾患モデル研究
  • 膵臓移植研究
  • 創薬毒性試験


Organoids Grown in PancreaCult™ Organoid Growth Medium (Mouse)

Pancreatic exocrine organoids are observed within one week when cultured in (A) Corning® Matrigel® domes or (B) a dilute Matrigel® suspension. Organoids were imaged during passage 2, on day 4.


Mouse Pancreatic Organoids can be Initiated from a Variety of Starting Materials

PancreaCult™ Organoid Growth Medium (Mouse) enables the initiation of pancreatic exocrine organoids from (A) duct fragments, (B) single cells and (C) cryopreserved organoid fragments. All organoids were grown in Matrigel® domes. Organoids were imaged on day 4 or day 5 of primary culture (duct fragments and single cells, respectively) or day 3 of the first passage post-thaw (cryopreserved organoids).


Pancreatic Organoids can be Grown in Matrigel® Domes or as a Dilute Matrigel® Suspension

Organoids cultured using PancreaCult™ Organoid Growth Medium (Mouse) from freshly isolated pancreatic tissue fragments and plated in (A) Matrigel® domes or (B) as a dilute Matrigel® suspension. Organoids grown in either culture condition are typically ready for passage within 3 - 6 days.


Pancreatic Exocrine Organoids Display Markers of Pancreatic Progenitor and Ductal Cells

Pancreatic exocrine organoids grown in PancreaCult™ and stained for nuclei (DAPI, blue), ductal marker KRT19 (green) and pancreatic progenitor marker PDX1 (red). Organoids were imaged during passage 12 on day 5.
Note: The folded appearance of epithelium is a function of cryosectioning and not representative of the shape of proliferating organoids.


Pancreatic Exocrine Organoids Retain Pancreatic Marker Expression During Passaging

Pancreatic organoids express stem cell markers and those typical of the pancreatic exocrine system, including (A) Axin2, (B) Krt19, (C) Muc1 and (D) Pdx1. Relative quantification (RQ) of each marker is reported relative to the 18S and TBP housekeeping genes and normalized to C57/Bl6 pancreatic tissue. Marker expression was assayed during early passages (passage 1-5) and late passages (passage 6-10).


Expansion of Organoids Grown in PancreaCult™ Organoid Growth Medium (Mouse)

Organoids cultured with PancreaCult™ Organoid Growth Medium (Mouse) show efficient growth over multiple passages. Cultures were split with an average split ratio of 1:16 at each passage.


Pancreatic Exocrine Organoids Provide a Model for Pancreatic Carcinomas

PancreaCult™ Organoid Growth Medium (Mouse) supports the growth of organoids from pancreatic carcinomas. Pancreatic ducts were isolated from KPC mice (Kras+/LSL-G12D; Trp53+/LSL-R172H; Pdx1-Cre) and cultured in PancreaCult™ Organoid Growth Medium (Mouse). Organoids were imaged on (A) day 4 of primary culture and (B) day three after the first passage. An activated KRAS genotype was retained in organoids during culture. Data used with permission from Dr. David Tuveson.







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