STEMCELL Technologies StemSpan StemSpan CD34+ Expansion Supplement
- 研究用
StemSpan™ CD34+ Expansion Supplement（10X）（ST-02691）は、ヒト臍帯血（CB）および骨髄（BM）から分離したCD34+細胞の増殖を選択的に促進させる培地サプリメントです。組み換えヒトサイトカイン（Flt3L、SCF、IL-3、IL-6、TPO）とその他の添加物を含む製剤です。
StemSpan™ CD34+ Expansion Supplementは、通常CD34+ヒト臍帯血（CB）細胞の7日間の液体培養で全有核細胞を約40倍に増殖させます。１週間後には約40％の培養細胞がCD34を発現し、最初のCD34+CB細胞が10倍以上に増殖したことになります。この増殖は、UM729などの低分子化合物の添加によってさらに効率を高めることが可能です。
StemSpan™ CD34+ Expansion Supplementは以下のStemSpan™培地と組み合わせた使用を想定しています。
・ StemSpan™ SFEM (ST-09600)
・ StemSpan™ SFEM II (ST-09605)
・ StemSpan™-XF (ST-100-0073)
・ StemSpan™-AOF (ST-100-0130)
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
ヒトCD34陽性造血細胞増殖のための無血清培養用サプリメントです
CD34+ Expansion Supplementを添加したStemSpan™ SFEM培地による、ヒト臍帯血CD34+細胞の拡大培養
Shown are the percent CD34+ cells, fold expansion of total nucleated cells (TNC) and CD34+ cells, and numbers of colony-forming units (CFU) produced per input CD34+ cell after 7 days of hsc expansion culture of enriched CD34+ cells from six independent cord blood (CB) samples.
*95% confidence limits, the range within which 95% of the results will typically fall. ND: not done
CD34+ Expansion Supplementを添加した異なるStemSpan培地での、CD34+細胞増殖の比較
Average expansion of (A) total nucleated cells (TNC), (B) CD34+ cells and (C) colony-forming units (CFU), normalized relative to the values obtained in StemSpan™ SFEM (grey bars) after culturing purified hematopoietic CD34+ cord blood cells (n=6) for 7 days in StemSpan™ SFEM, SFEM II (blue bars) or AOF (orange bars) media containing CD34+ Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results will typically fall. Cell yields in StemSpan™ SFEM II were on average ~60% higher than in StemSpan™ SFEM and StemSpan™-AOF. *p<0.001, #p<0.05 (paired t-test, n=6 in A and B, n=4 in C).
Note: Data for StemSpan™-AOF shown were generated with the original phenol red-containing version StemSpan™-ACF (Catalog #09855). However internal testing showed that the performance of the new phenol red-free, cGMP-manufactured version, StemSpan™-AOF (Catalog #100-0130) was comparable.
