FAQ
- 用途別細胞培養
- PneumaCult-ALIで培養した細胞をPAS染色する際のプロトコルは有りますか？
-
STEMCELL technologies社では、PAS染色を下記のようなプロトコルで行っています。
Fixation: 10% formalin.
Section: paraffin sections at 3-5 um.
Solutions and Reagents:
0.5% Periodic Acid Solution: Periodic acid - 0.5 g, Distilled water - 100 mL
Procedure:
1. Deparaffinize and hydrate to water.
2. Oxidize in 0.5% periodic acid solution for 5 minutes.
3. Rinse in distilled water.
4. Place in Schiff reagent for 15 minutes (Sections become light pink color during this step).(From Sigma-Aldrich)
5. Wash in lukewarm tap water for 5 minutes (Immediately sections turn dark pink color).
6. Counter stain in Gills hematoxylin for 1 minute.
7. Wash in tap water for 5 minutes.
8. Dehydrate and coverslip using a synthetic mounting medium.
Results:
Glycogen mucin and some basement membranes --- red/purple
Fungi --- red/purple
Background --- blue
