STEMCELL Technologies ImmunoCult ImmunoCult Mouse B Cell Expansion Kit
- 研究用
ImmunoCult™ Mouse B Cell Expansion Kit（ST-100-1003）は、煩雑なフィーダー細胞を使用することなく、無血清条件でマウスB細胞を一貫して拡大培養するためのキットです。
本キットは、マウス脾臓細胞からB細胞を高収率で増殖するために最適な培養条件を提供いたします。ImmunoCult™-XF B Cell Base MediumとImmunoCult™ ACF Mouse B Cell Expansion Supplementから構成されており、堅牢で使い勝手の良い培養系です。わずか6～9日間の培養後に細胞を回収し、下流のアプリケーションにそのまま使用することができます。
STEMCELL Technologies社の他の上流および下流製品との互換性があります。たとえば、EasySep™ Mouse Pan-B Cell Isolation Kit（ST-19844）で分離したB細胞を、本キットですぐに増殖できます。
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
ImmunoCult™ Mouse B Cell Expansion Kitをもちいて、無血清条件下でマウスB細胞を効率よく増殖できます！
- マウスB細胞のin vitroでの増殖を、血清やフィーダー細胞、専用プレートを使用せずに実施できます
- 最適化された使いやすい培養系で、ワークフローをシンプルにします
- 拡大培養したマウスB細胞を、一貫した収量で取得できます
B細胞培養の流れ
Figure 1. Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured in complete Mouse B Cell Expansion Medium as described in Directions For Use (steps 1 - 7). B Cells were harvested on day 9 for analysis. Cells can also be harvested at earlier time points depending on different applications.
データ紹介
マウスB細胞の増殖
Figure 2. Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Fold expansion of viable cells is shown with bar graphs representing the mean ± SEM (n = 8). B cells expanded 176.9 ± 29.8-fold after 9 days of culture.
マウスB細胞の成熟
Figure 3. Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. A) Expression of B220 and CD138 and B) Expression of TACI (CD267) and CD86 were analyzed by flow cytometry at each timepoint (data represents mean ± SEM, n = 8). An increase in CD86 cell surface expression indicates B cell activation; a decrease in B220 and an increase in CD138 and TACI cell surface expression indicates maturation of B cells to plasmablasts or plasma cells.
マウスB細胞の形態
Figure 4. Robust Growth of Mouse B Cells when Cultured with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Cells were imaged at A) 10X magnification on day 0, B) 10X magnification on day 3, C) 40X magnification on day 6, and D) 40X magnification on day 9.
