2019/08/19

Watch as cells grow - Culture ES and iPS cells on laminin

Stem cell culturing of human ES and iPS cells on recombinant laminin matrices is easy and efficient. Human ES and iPS cells grow as monolayers in a completely defined and animal component-free cell culture environment that enables self-renewal and pluripotency without the use of ROCK inhibitors. Here, we show hES cells plated as single cells on top of recombinant laminin-521, growing from day 0 to day 5 when they are ready to be split again. During the first 1-2 days, a picture was taken every 15 minutes. Days 3-5 a picture was taken every hour.