FAQ
- 用途別細胞培養
- ImmunoCult Dendritic Cell Culture Kitは株化細胞からの樹状細胞分化に使用できますか?
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ImmunoCult Dendritic Cell Culture Kitを用いた単球系株化細胞(THP-1, U937を含む)からの樹状細胞分化については、あいにく情報がございません。試していただく価値はございますが、性能保証はいたしかねます。
文献には次のような記載もございます。
- It seems THP-1 only show a very low DC differentiation capacity when only stimulated with cytokines, but differentiation does work when calcium ionophores such as ionomycin are added to the cytokine supplements.
- On the other hand, the U937 cell line is not able to differentiate into DCs.
参考文献:
- Saskia J. A. M. Santegoets et al. Human dendritic cell line models for DC differentiation and clinical DC vaccination studies. JLB 2008
- Chanput et al. THP-1 and U937 Cells. The Impact of Food Bioactives on Health pp 147-159