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  • タンパク・遺伝子発現解析

  • Dynabeadsで「原形質膜」を分離した例、「リソソーム」を分離した例は有りますか?
  • 原形質膜、リソソームそれぞれを分離した例をご紹介します。
    下記の文献をご参照ください。


    原形質膜の分離例 :


    Madey E1, et al. Characterization of plasma membrane domains enriched in lipid metabolites. J Exp Bot. 2001 Apr;52(357):669-79.





    リソソームの分離例:

    Vitale N1, et al. Localization of ADP-ribosylation factor domain protein 1 (ARD1) in lysosomes and Golgi apparatus. Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8613-8.






    Dynabeads used for exosome isolation via an antibody plus some other references where Dynabeads M-500 Subcellular has been used to isolate other types of organelles.
    ※ Dynabeads M-500 Subcellularは販売中止となっております。代替品はDynabeads M-450 Tosylactivated(製品コード:DB14013)です。


    ? H. Rabesandratana J. P. Toutant, H. Reggio, and M. Vidal. Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during in vitro maturation of reticulocytes. Blood 91:2573-2580 1998.


    ? B. B. Allan, B. D. Moyer and W. E. Balch. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science 289 (5478):444-448 2000.


    ? X. Li, T. Galli, S. Leu, J. B. Wade, E. J. Weinman, G. Leung, A. Cheong, D. Louvard and M. Donowitz. Na+-H+ exchanger 3 (NHE3) is present in lipid rafts in the rabbit ileal brush border: a role for rafts in trafficking and rapid stimulation of NHE3. J.Physiol 537 (Pt 2):537-552, 2001.



    Some references for isolation of endosomes and lysosomes.

    ? R. Gagescu, N. Demaurex, N. Demaurex, R. G. Parton, W. Hunziker, L. A. Huber and J. Gruenberg. The recycling of endosome of madin-darby canine kidney cells is a mildly acidic compartment rich in raft components. MOLECULAR BIOLOGY OF THE CELL 11:2775-2791, 2000.



    ? C. J. Provoda, M. T. Waring and K. M. Buckley. Evidence for a primary endocytic vesicle involved in synaptic vesicle biogenesis. Journal of Biological Chemistry 275 (10):7004-7012 2000.




    ? T. E. Tjelle, B. Saigal, M. Froystad and T. Berg. Degradation of phagosomal components in late endocytic organelles. J.Cell Sci. 111:141-148 1998.




    ? N. Vitale, K. Horiba, V. J. Ferrans, J. Moss and M Vaughan. Localization of ADP-ribosylation factor domain protein (ARD1) in lysosomes and Golgi apparatus. Proc.Natl.Acad.Sci.USA 95:8613-8618 1998.





    Some references to articles where vesicles transporting from ER has been isolated + some other interesting references regarding same topic:




    ? B. B. Allan, B. D. Moyer and W. E. Balch. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science 289 (5478):444-448 2000.




    ? J. Seemann, M. Pypaert, T. Taguchi, J. Malsam and G. Warren. Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells. Science 295 (5556):848-851 2002.




    ? S. A Jesch and A. D Linstedt. The golgi and endoplasmic reticulum remain independent during mitosis and HeLa cells. MOLECULAR BIOLOGY OF THE CELL 9:623-635 1998.




    ? P Sinai, P Webster and K. A Joiner. Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction. JOURNAL OF CELL SCIENCE 110:2117-2128 1997.




    ? N. Sans, C. Racca, R. S. Petralia, Y-X. Wang, J. McCallum and R. J. Wenthold. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. JOURNAL OF NEUROSCIENCE 21 (19):7506-7516 2001.




    ? K. A. H. Abo-Hashema, M. H. Cake, G. W Power and D. Clarke. Evidence for triacylglycerol synthesis in the lumen of microsomes via a lypolysis-esterification pathway involving carnitine acyltransferases. Journal of Biological Chemistry 274 (50):35577-35582 1999.





    References where Dynabeads have been used to isolate mitochondria and other organelles.




    ? G. H. L?ers  R. Hartig, H. Mohr, M. Hausmann, H. D. Fahimi, C. Cremer and A. V?lkl. Immuno-isolation of highly purified peroxisomes using magnetic beads and continuous immunomagnetic sorting. Electrophoresis 19:1205-1210 1998.




    ? P. S. Sexton and R. J. Cenedella. Immunomagnetic capture of lens membrane fractions containing steroid binding protein. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 295 (4):1027-1031   2002. ? N. Sans, C. Racca, R. S. Petralia, Y-X. Wang, J. McCallum and R. J. Wenthold. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. JOURNAL OF NEUROSCIENCE 21 (19):7506-7516 2001.




    ? G. H. Hansen, L Immerdal, E. Thorsen, L-L. Niels-Christiansen, B. T. Nystr?m E. J. F. Demant and E. M. Danielsen. Lipid Rafts Exist as Stable Choloesterol-independent Microdomains in the Brush Border Membrane of Enterocytes. J.Biol.Chem. 276 :32338-32344 2002.




    ? P Sinai, P Webster and K. A Joiner. Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction. JOURNAL OF CELL SCIENCE 110:2117-2128 1997.




    ? S. M. Jones, R. H. Dahl, J. Ugelstad and K. E. Howell. Immunoisolation of organelles using magnetic solid supports. In: Cell Biology: A laboratory handbook Anonymous Academic press, 1998 p. 12-25.




    ? A M?sch, D Cohen and E. Rodriquez-Boulan. Myosin II is involved in the production of constitutive transport vesicles from the TGN. Journal of Cell Biology 138 (2):291-306 1997.




    ? J. Provoda, M. T. Waring, and K. M. Buckley. Evidence for a primary endocytic vesicle involved in synaptic vesicle biogenesis. Journal of Biological Chemistry 275 (10):7004-7012, 2000



    原形質膜の分離例 :

    Madey E1, et al. Characterization of plasma membrane domains enriched in lipid metabolites. J Exp Bot. 2001 Apr;52(357):669-79.


    リソソームの分離例:

    Vitale N1, et al. Localization of ADP-ribosylation factor domain protein 1 (ARD1) in lysosomes and Golgi apparatus. Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8613-8.



    Dynabeads used for exosome isolation via an antibody plus some other references where Dynabeads M-500 Subcellular has been used to isolate other types of organelles.
    ※ Dynabeads M-500 Subcellularは販売中止となっております。代替品はDynabeads M-450 Tosylactivated(製品コード:DB14013)です。

    ? H. Rabesandratana J. P. Toutant, H. Reggio, and M. Vidal. Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during in vitro maturation of reticulocytes. Blood 91:2573-2580 1998.

    ? B. B. Allan, B. D. Moyer and W. E. Balch. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science 289 (5478):444-448 2000.

    ? X. Li, T. Galli, S. Leu, J. B. Wade, E. J. Weinman, G. Leung, A. Cheong, D. Louvard and M. Donowitz. Na+-H+ exchanger 3 (NHE3) is present in lipid rafts in the rabbit ileal brush border: a role for rafts in trafficking and rapid stimulation of NHE3. J.Physiol 537 (Pt 2):537-552, 2001.


    Some references for isolation of endosomes and lysosomes.

    ? R. Gagescu, N. Demaurex, N. Demaurex, R. G. Parton, W. Hunziker, L. A. Huber and J. Gruenberg. The recycling of endosome of madin-darby canine kidney cells is a mildly acidic compartment rich in raft components. MOLECULAR BIOLOGY OF THE CELL 11:2775-2791, 2000.
    ? C. J. Provoda, M. T. Waring and K. M. Buckley. Evidence for a primary endocytic vesicle involved in synaptic vesicle biogenesis. Journal of Biological Chemistry 275 (10):7004-7012 2000.

    ? T. E. Tjelle, B. Saigal, M. Froystad and T. Berg. Degradation of phagosomal components in late endocytic organelles. J.Cell Sci. 111:141-148 1998.

    ? N. Vitale, K. Horiba, V. J. Ferrans, J. Moss and M Vaughan. Localization of ADP-ribosylation factor domain protein (ARD1) in lysosomes and Golgi apparatus. Proc.Natl.Acad.Sci.USA 95:8613-8618 1998.


    Some references to articles where vesicles transporting from ER has been isolated + some other interesting references regarding same topic:

    ? B. B. Allan, B. D. Moyer and W. E. Balch. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science 289 (5478):444-448 2000.

    ? J. Seemann, M. Pypaert, T. Taguchi, J. Malsam and G. Warren. Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells. Science 295 (5556):848-851 2002.

    ? S. A Jesch and A. D Linstedt. The golgi and endoplasmic reticulum remain independent during mitosis and HeLa cells. MOLECULAR BIOLOGY OF THE CELL 9:623-635 1998.

    ? P Sinai, P Webster and K. A Joiner. Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction. JOURNAL OF CELL SCIENCE 110:2117-2128 1997.

    ? N. Sans, C. Racca, R. S. Petralia, Y-X. Wang, J. McCallum and R. J. Wenthold. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. JOURNAL OF NEUROSCIENCE 21 (19):7506-7516 2001.

    ? K. A. H. Abo-Hashema, M. H. Cake, G. W Power and D. Clarke. Evidence for triacylglycerol synthesis in the lumen of microsomes via a lypolysis-esterification pathway involving carnitine acyltransferases. Journal of Biological Chemistry 274 (50):35577-35582 1999.


    References where Dynabeads have been used to isolate mitochondria and other organelles.

    ? G. H. L?ers  R. Hartig, H. Mohr, M. Hausmann, H. D. Fahimi, C. Cremer and A. V?lkl. Immuno-isolation of highly purified peroxisomes using magnetic beads and continuous immunomagnetic sorting. Electrophoresis 19:1205-1210 1998.

    ? P. S. Sexton and R. J. Cenedella. Immunomagnetic capture of lens membrane fractions containing steroid binding protein. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 295 (4):1027-1031   2002. ? N. Sans, C. Racca, R. S. Petralia, Y-X. Wang, J. McCallum and R. J. Wenthold. Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway. JOURNAL OF NEUROSCIENCE 21 (19):7506-7516 2001.

    ? G. H. Hansen, L Immerdal, E. Thorsen, L-L. Niels-Christiansen, B. T. Nystr?m E. J. F. Demant and E. M. Danielsen. Lipid Rafts Exist as Stable Choloesterol-independent Microdomains in the Brush Border Membrane of Enterocytes. J.Biol.Chem. 276 :32338-32344 2002.

    ? P Sinai, P Webster and K. A Joiner. Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction. JOURNAL OF CELL SCIENCE 110:2117-2128 1997.

    ? S. M. Jones, R. H. Dahl, J. Ugelstad and K. E. Howell. Immunoisolation of organelles using magnetic solid supports. In: Cell Biology: A laboratory handbook Anonymous Academic press, 1998 p. 12-25.

    ? A M?sch, D Cohen and E. Rodriquez-Boulan. Myosin II is involved in the production of constitutive transport vesicles from the TGN. Journal of Cell Biology 138 (2):291-306 1997.

    ? J. Provoda, M. T. Waring, and K. M. Buckley. Evidence for a primary endocytic vesicle involved in synaptic vesicle biogenesis. Journal of Biological Chemistry 275 (10):7004-7012, 2000

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