FAQ
- タンパク・遺伝子発現解析
- ストレプトアビジン結合Dynabeadsを用いて「Nucleic acid capture assay」を行った例は有りますか？
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Dynabeadsを用いたNucleic acid capture assayについてメーカー側が推奨するプロトコールは特にありませんが、下記の論文例がありますのでご参考下さい。
Dynabeads Streptavidinを用いたNucleic acid capture assayの論文例
Meng Q. et al. (2001). Automated multiplex assay system for simultaneous detection of hepatitis B virus DNA, hepatitis C virus RNA and human immunodeficiency virus type 1 RNA. J.Clin.Microbiol. 39(8):2937-2945.
Pollock GS. et al. (2001). Effects of early visual experience and diurnal rhythms on BDNF mRNA and protein levels in the visual system, hippocampus and cerebellum. J. Neurosci. 21(11):3923-3931.
Miyashiro I. et al. (2001). Molecular strategy for detecting metastatic cancers with use of multiple tumor-specific MAGE-A genes. Clin. Chem. 47(3):505-512.
Stevens SJC. et al. (1999). Monitoring of Epstein-Barr virus DNA load in peripheral blood by quantitative competitive PCR. J.Clin. Microbiol. 37:2852-2857.
Mangiapan G. et al. (1996). Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens. J. Clin. Microbiol. 34(5): 1209-1215.
Dong SM. et al. (2001). Detection of colorectal cancer in stool with the use of multiple genetic targets. J Natl Cancer Inst. 93(11): 858-865.
Refseth UH. et al. (1997). Hybridization capture of microsatellites directly from genomic DNA. Electrophoresis. 18(9):1519-1523.