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ヒトMammosphere培養用培地 MammoCult

[ 2010-04-01 ]

 

Mammosphere（マンモスフィア）の培養

 

MammoCult Medium

MammoCult Medium（Human）は、ヒト乳腺幹細胞/前駆細胞をMammosphereとして

培養するためのdefinedな培地です。組成はhuman mammary progenitorsが溶液中で

生存、増殖することを促進するように最適化されており、系統的制限、二分化能前駆細胞

をCFC（コロニー形成細胞）アッセイで検出することが可能です。

Mammmosphereアッセイは、in vitroでの乳腺幹細胞/前駆細胞の評価方法として乳がん

幹細胞研究の分野で幅広く使われるようになっています。

 

 

 

MammoCult 使用概要

 

 

 

 

 

MammoCult Mediumの特長

 

  • 無血清、Definedな培地組成
  • 文献で紹介されている従来の組成（Dontuの組成）と比較して11倍のmammosphere形成ユニット数を passage 3で実現、Passage 1でも最大2倍の数のmammosphereを形成
  • 乳腺前駆細胞が従来の組成よりも保存される
  • 従来の組成培地と比較してより大きなmammosphereを多数形成 

 

 

 

 

 

MammosphereおよびTumorsphere培養用培地MammoCult 

 

 

 

MammoCultはプライマリーなヒト乳腺上皮細胞および多数の乳がんセルライン（MDA-MB-231、SUM149、SK-BR3、MCF7など）の成長をサポートする唯一の市販培地です。

 

 

 

 

Mammosphere

 

付着非依存性：MammosphereとTumosphere


 
付着依存性:3D Morphogenesis Assay

EpiCult-B + Matrigel™


 

 

 

Mammosphereアッセイのアプリケーション

 

  • 正常ヒト乳腺幹細胞のキャラクタライズ
  • がん性のヒト乳腺幹細胞のキャラクタライズ
  • ヒト乳がん細胞株の評価：幹細胞性、遺伝子機能、タンパク質機能、シグナル経路
  • ドラッグディスカバリー、毒性試験
  • その他

 

 

製品一覧

 

製品コード   
 品名      容量   
ST-05620   
 MammoCult Medium (Human) 500 mL
ST-07904   
 Hydrocortisone 100 mg
ST-07980   
 0.2% Heparin solution 2 mL

 

 

製品構成（ST-05620）

 

・ MammoCult™ Basal Medium (Human): 450 mL
・ MammoCult™ Proliferation Supplements (Human): 50 mL
＊使用には別途Hydrocortisone とHeparinを添加する必要がございます。

 

 

製品資料

 

 

 

乳腺幹細胞関連製品

 

・ALDEFLUOR：ALDH活性を利用した幹細胞の濃縮
乳腺幹細胞/前駆細胞の濃縮のためにALDHの活性を利用した幹細胞同定方法が広く用いられるようになってきています。ALDH活性の高い幹細胞をmammosphereアッセイで評価することも可能です。以下は参考文献です。

Mammary Epithelial-Specific Ablation of the Focal Adhesion Kinase Suppresses Mammary Tumorigenesis by Affecting Mammary Cancer Stem/Progenitor Cells Ming Luo, Huaping Fan, Tamas Nagy, Huijun Wei, Chenran Wang, Suling Liu, Max S. Wicha, and Jun-Lin Guan


BRCA1 regulates human mammary stem/progenitor cell fate Suling Liu, Christophe Ginestier, Emmanuelle Charafe-Jauffret, Hailey Foco, Celina G. Kleer, Sofia D. Merajver, Gabriela Dontu, and Max S. Wicha


DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast J. Dinny Graham, Patricia A. Mote, Usha Salagame, Jessica H. van Dijk, Rosemary L. Balleine, Lily I. Huschtscha, Roger R. Reddel, and Christine L. Clarke


Altered Proliferation and Differentiation Properties of Primary Mammary Epithelial Cells from BRCA1 Mutation Carriers Laura N. Burga, Nadine M. Tung, Susan L. Troyan, Mihnea Bostina, Panagiotis A. Konstantinopoulos, Helena Fountzilas, Dimitrios Spentzos, Alexander Miron, Yosuf A. Yassin, Bernard T. Lee, and Gerburg M. Wulf

 

 

Mammosphereアッセイに関する参考文献

 

1.
Characterization of non-adherent mammosphere cultures of normal human breast tissue Agla J. Fridriksdottir, Rene Villadsen, Thorarinn Gudjonsson, Lone Ronnov-Jessen, and Ole W. Petersen[Normal breast tissue]

2.
Enrichment of a Population of Mammary Gland Cells that Form Mammospheres and Have In vivo Repopulating Activity Mai-Jing Liao, Cheng Cheng Zhang, Beiyan Zhou, Drazen B. Zimonjic, Sendurai A. Mani, Megan Kaba, Ann Gifford, Ferenc Reinhardt, Nicholas C. Popescu, Wenjun Guo, Elinor Ng Eaton, Harvey F. Lodish, and Robert A. Weinberg[Normal breast tissues]


3.
Identification of Tumor-Initiating Cells in a p53-Null Mouse Model of Breast Cancer Mei Zhang, Fariba Behbod, Rachel L. Atkinson, Melissa D. Landis, Frances Kittrell, David Edwards, Daniel Medina, Anna Tsimelzon, Susan Hilsenbeck, Jeffrey E. Green, Aleksandra M. Michalowska, and Jeffrey M. Rosen[p53 loss of function]

4.
Mammary Epithelial-Specific Ablation of the Focal Adhesion Kinase Suppresses Mammary Tumorigenesis by Affecting Mammary Cancer Stem/Progenitor Cells Ming Luo, Huaping Fan, Tamas Nagy, Huijun Wei, Chenran Wang, Suling Liu, Max S. Wicha, and Jun-Lin Guan[ALDH+cells and mammosphere assay]

5.
Breast Cancer Cell Lines Contain Functional Cancer Stem Cells with Metastatic Capacity and a Distinct Molecular Signature Emmanuelle Charafe-Jauffret, Christophe Ginestier, Flora Iovino, Julien Wicinski, Nathalie Cervera, Pascal Finetti, Min-Hee Hur, Mark E. Diebel, Florence Monville, Julie Dutcher, Marty Brown, Patrice Viens, Luc Xerri, François Bertucci, Giorgio Stassi, Gabriela Dontu, Daniel Birnbaum, and Max S. Wicha[Human breast cancer cell lines]

6.
Inhibition of Notch signaling reduces the stem-like cell population in T47D breast cancer cells and prevents mammosphere formation. P Grudzien, P Strack, T Golde, L Miele, and KE Foreman[Notch signaling]

7.
Novel Cell Culture Technique for Primary Ductal Carcinoma In Situ: Role of Notch and Epidermal Growth Factor Receptor Signaling Pathways Gillian Farnie, Robert B. Clarke, Katherine Spence, Natasha Pinnock, Keith Brennan, Neil G. Anderson, and Nigel J. Bundred[Notch and EGF Signaling]

8.
Hedgehog Signaling and Bmi-1 Regulate Self-renewal of Normal and Malignant Human Mammary Stem Cells Suling Liu, Gabriela Dontu, Ilia D. Mantle, Shivani Patel, Nam-shik Ahn, Kyle W. Jackson, Prerna Suri, and Max S. Wicha[Hedgehog and Bmi-1 signaling]

9.
Cancer stem cell inhibitor identified using a mammosphere-based in vitro assay Kirsty Holden, Shaun Ainsworth, Matthew Ablett, David Ansell, Jeffrey Moore, Joanne Read, Patricia Hurley, Robert Clarke, Catherine Booth, and Gerard Brady[Drug discovery]

10.
Intrinsic Resistance of Tumorigenic Breast Cancer Cells to Chemotherapy Xiaoxian Li, Michael T. Lewis, Jian Huang, Carolina Gutierrez, C. Kent Osborne, Meng-Fen Wu, Susan G. Hilsenbeck, Anne Pavlick, Xiaomei Zhang, Gary C. Chamness, Helen Wong, Jeffrey Rosen, and Jenny C. Chang[Therapeutics]

11.
Curcumin and piperine may prevent breast cancer by modulating stem cell fate Madhuri Kakarala, Dean Brenner, Max Wicha, and Gabriela Dontu[Drug discovery]