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Dynabeadsでマウス内皮細胞を分離している例を教えてください。

下記文献をご参考下さい。


・Nucleic Acids Res. (2004); 32(2): 757-766.

 Mouse heart endothelial cells isolated using rat anti-mouse CD31 (clone MEC13.3, BD Pharmingen) and Dynabeads M-450 Sheep anti-Rat IgG.

 

・ Laboratory Investigation (2004) 84, 1472-1483

 Mouse endothelial cells isolated using rat anti-mouse CD31 (clone ER-MP12) and Dynabeads M-450 Sheep anti-Rat IgG.

 

・ Blood (2003); 102(9):3224-31

 Mouse ECs isolated using Dynabeads M-450 Sheep anti-Rat IgG and a rat anti-mouse ICAM-2 (CD102) antibody (clone3C4?) from PharMingen.

 

・ Am J Pathol. (2003);162(5):1591-601

 Dynabeads Sheep anti-Rat IgG were coated with either anti-PECAM-1 (CD31) or anti-ICAM-2 (CD102) monoclonal antibody (2.5 μg antibody for 2 x 107 beads) and used for isolation of mouse lung or heart ECs.

 

・ J Biol Chem. (2002);277(18):16081-7.

 Mouse ECs isolated using Dynabeads coated with a rat anti-mouse ICAM-2 (CD102) antibody (BD Pharmingen). Purity >90%

 

・ Mol Biol Cell. (2002);13(6):1893-905

 Mouse lung ECs isolated using Dynabeads Sheep anti-Rat IgG and a BD Pharmingen rat anti-mouse ICAM-2 (CD102) antibody.

 

・ Circ Res. (2002);91:70-76

 Mouse lung endothelial cells were labelled with a Rat anti-Mouse PECAM-1 (CD31) antibody (BD Pharmingen), and endothelial cells isolated using the Dynabeads M-450 Sheep anti-Rat IgG. Isolated cells washed, detached from the beads using trypsin treatment for 3 minutes and put in culture.

 

・ Proc Natl Acad Sci (2000); 97(23): 12782-12787

 Dynabeads M-450 Sheep anti- Rat IgG and rat anti-mouse E-selectin (CD62E, BD Pharmingen) used for isolation of mouse pulmonary (lung) ECs.

In addtion please find below:
1.    Dynabeads endothelial cell isolation protocol from Harvard
Isolation of Mouse Lung Endothelial Cells (MLEC)/ Mouse Heart Endothelial Cells (MHEC) from Harvard Medical School
http://vrd.bwh.harvard.edu/core_facilities/mlec.html

2.    Dynabeads visualized isolation
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice: http://www.jove.com/details.php?id=2316 Using Dynabeads secondary coated


回答日:2014/03/10

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DynabeadsCD31、マウス内皮細胞 二次抗体結合Dynabeads