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Dynabeadsのアセトンに対する耐性について教えてください。

ビーズ自体は、アセトンに耐性がございます。表面にコートされているものがストレプトアビジンの場合、アセトンのようなケトン類は、避ける必要があります。また、Protein A/Gの場合について、情報はございません。 また、アセトンのタンパク質への影響については、アセトンをご購入頂いた試薬メーカーにご確認下さい。
The beads themselves are compatible with a number of solvents, such as: - ethanol (70% is actually used to sterilize the beads/washings), - methanol, - acetone, - isopropanol, - diglym, - DMSO (dimethyl sulfoxide) - DMF (dimethylformamide). - THF (tetrahydrofuran) is similar to diglym and should work fine with Dynabeads All these will leave the iron oxide precipitates untouched. - chlorinated solvents may cause some swelling of the beads Furthermore, working with acetonitrile is without problem for the beads, but dichloromethane (CH2Cl2) may release organic polymers from the bead. Two important factors for all solvents are concentration and incubation time. The surfaces of the different beads gives some restrictions. Streptavidin surface: ketones like acetone should be avoided. 50% DMF is not compatible with streptavidin (it may dissociate into subunits, up to 20% might be ok) (Please note: the organic solvents will not harm the beads themselves, it is the streptavidin that is the problem) Regarding Dynabeads Protein A/G we do not have any information whether these beads are compatible with acetone.

回答日:2011/10/31

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アセトン耐性